{"id":166,"date":"2014-04-28T16:03:45","date_gmt":"2014-04-28T16:03:45","guid":{"rendered":"https:\/\/www.clgenetics.com\/wp\/?page_id=166"},"modified":"2014-05-07T18:12:31","modified_gmt":"2014-05-07T18:12:31","slug":"str-profiling-200","status":"publish","type":"page","link":"https:\/\/clgenetics.com\/wp\/our-services\/short-tandem-repeat-analysis\/str-profiling-200\/","title":{"rendered":"STR Profiling 200"},"content":{"rendered":"<div class=\"cms_content\">\n<p>\t<img loading=\"lazy\" src=\"https:\/\/www.clgenetics.com\/wp\/wp-content\/uploads\/2014\/04\/stranalysis.jpg\" alt=\"GBAND flat\" width=\"949\" height=\"222\" \/><\/p>\n<h2 class=\"style2\" style=\"font-size: 17px;\"><em>STR Profiling Services for Stem Cell Research and Regenerative Medicine<\/em><\/h2>\n<h2>STR Profiling 200<\/h2>\n<p><strong><em>Routine QA assay to detect profile drift, mix-ups and cross contamination via STR comparison analysis<\/em><\/strong>\u00a0<\/p>\n<p>\tSTR200 is a quality assurance STR profile assay intended for previously authenticated cell lines. The test measures identity and detects cell line mix-ups and cross contamination by comparing a cell line\u2019s current and past STR profiles.\u00a0 Cell Line Genetics recommends STR200 as a routine QA service to confirm and\/or monitor a cell lines identity at different passages and after large experiments.\u00a0<br \/>\n\t\u00a0<\/p>\n<p>STR200 may also be used to compare a sample\u2019s STR profile against any other STR profile to determine percentage of identity between two lines.\u00a0 STR300 is the recommended precursor service to STR200.<\/p>\n<div class=\"you-tabs\">\n<div class=\"tab tabc1 active-tab\"><a class=\"tabref tab1\" href=\"#\" data-tab=\"1\">Attributes<\/a><\/div>\n<div class=\"tab tabc2 \"><a class=\"tabref tab2\" href=\"#\" data-tab=\"2\">Service Specs<\/a><\/div>\n<div class=\"tab tabc3\"><a class=\"tabref tab3\" href=\"#\" data-tab=\"3\">Sample Requirements<\/a><\/div>\n<\/p><\/div>\n<div class=\"you-box you-box1\">\n<ul style=\"padding-left:40px;\">\n<li>PowerPlex&#8482; 16 System for co-amplification and three color detection of fifteen STR loci and Amelogenein\n<\/li>\n<li>Complete STR profile report including allele table and electropherogram\n<\/li>\n<li>Culture swabs, isolated DNA, and live cells accepted\n<\/li>\n<li>DNA extraction and amplification (additional $30.00)\n<\/li>\n<li>Cross contamination detection and analysis\n<\/li>\n<li>STR profile comparison analysis\n<\/li>\n<li>Comprehensive interpretation by a Cell Line Genetics senior scientist\n<\/li>\n<li>Results securely stored in CLG database\n<\/li>\n<li>Fast turnaround time, highly accurate, competitively priced\n<\/li>\n<\/ul><\/div>\n<div class=\"you-box you-box2 hidden\">\n<table cellpadding=\"0\" cellspacing=\"2\">\n<tr>\n<td width=\"25%\" align=\"left\" valign=\"top\" bgcolor=\"#ccccff\" style=\"padding:5px 5px 15px 5px;\">\n<h3>Used to:<\/h3>\n<\/td>\n<td width=\"75%\" align=\"left\" valign=\"top\" bgcolor=\"#ccccff\" style=\"padding:5px 5px 15px 5px;\">\n<p>- Authenticate existing cell lines<br \/>\n\t\t        <br \/>\n\t\t        - Determine the relationship between two cell lines at different    passages <\/p>\n<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" valign=\"top\" bgcolor=\"#e9edf4\" style=\"padding:5px 5px 15px 5px;\">\n<h3>Detects:<\/h3>\n<\/td>\n<td align=\"left\" valign=\"top\" bgcolor=\"#e9edf4\" style=\"padding:5px 5px 15px 5px;\">\n<p>- STRs to confirm or determine origin of a cell line by matching the STRs to an existing STR profile for a cell line authentication purposes<br \/>\n\t\t          <br \/>\n\t\t     - Presence of more than one cell line of the same species (cross contamination) <\/p>\n<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" valign=\"top\" bgcolor=\"#ccccff\" style=\"padding:5px 5px 15px 5px;\">\n<h3>Doesn&rsquo;t  Detect:<\/h3>\n<\/td>\n<td align=\"left\" valign=\"top\" bgcolor=\"#ccccff\" style=\"padding:5px 5px 15px 5px;\">\n<p>- Cell line cross contamination involving non-human species<br \/>\n\t\t        <br \/>\n\t\t     - Cross contamination of cell lines derived from the same parental line <\/p>\n<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" valign=\"top\" bgcolor=\"#e9edf4\" style=\"padding:5px 5px 15px 5px;\">\n<h3>Sample Requirements:<\/h3>\n<\/td>\n<td align=\"left\" valign=\"top\" bgcolor=\"#e9edf4\" style=\"padding:5px 5px 15px 5px;\">\n<p>Live cell culture (T-25 flask)<br \/>\n\t\t      DNA<br \/>\n\t\t    Frozen Cells<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" valign=\"top\" bgcolor=\"#ccccff\" style=\"padding:5px 5px 15px 5px;\">\n<h3>Species:<\/h3>\n<\/td>\n<td align=\"left\" valign=\"top\" bgcolor=\"#ccccff\" style=\"padding:5px 5px 15px 5px;\">\n<p>Human<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" valign=\"top\" bgcolor=\"#e9edf4\" style=\"padding:5px 5px 15px 5px;\">\n<h3>Turnaround:<\/h3>\n<\/td>\n<td align=\"left\" valign=\"top\" bgcolor=\"#e9edf4\" style=\"padding:5px 5px 15px 5px;\">\n<p>7 - 10 business days <br \/>\n\t\t    RUSH services available<\/p>\n<\/td>\n<\/tr>\n<\/table><\/div>\n<div class=\"you-box you-box3 hidden\">\n<p><strong>PROCEDURE: Mailing Samples for Human DNA Fingerprinting<\/strong> [<a href=\"#\" target=\"_blank\">Print Sample Requirements<\/a>]<\/p>\n<p>&nbsp;<\/p>\n<p><strong>  Specimen Requirement<\/strong><br \/>\n\t\t  Human samples for DNA fingerprinting can be submitted in the form of culture swabs, isolated DNA, cells stored in liquid nitrogen, or live cells.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Requisition Form<\/strong><br \/>\n\t\t  Each sample must be accompanied by its own Test Requisition Form. The test requisition form can be <a href=\"https:\/\/www.clgenetics.com\/docs\/gallery\/downloads\/General-Requisition-Form.pdf\" target=\"_blank\">downloaded here<\/a>.<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"color: #FF0000; font-weight: bold;\">CAUTION<\/span><br \/>\n\t\t  <em>All personnel preparing samples for DNA fingerprinting should wear gloves to reduce the possibility of contamination with their own DNA.<\/em><\/p>\n<p>&nbsp;<\/p>\n<p><strong>To Send Culture Swabs from adherent cell cultures<\/strong><\/p>\n<p>&nbsp;<\/p>\n<ul style=\"padding-left:40px;\">\n<li>Wearing gloves, remove media from live cell culture.\n<\/li>\n<li>  Insert sterile cotton swab applicator (FISHER cat# 23-400-124) into the culture dish and scrape off a 1\u201d by 1\u201d area of<br \/>\n\t\t  cells<\/p>\n<\/li>\n<li>Place the swab in a sterile 1.5mL microcentrifuge tube and break off the shaft 0.25\u201d above the swab.\n<\/li>\n<li>Close the lid and label the tube with a unique cell line identifier.\n<\/li>\n<li>Prepare a duplicate swab and place in a separate 1.5mL microcentrifuge tube and label with a unique cell line identified.\n<\/li>\n<li>When ready to ship, fill out a requisition form for each sample and place the form and both tubes in a ziplock bag, wrap<br \/>\n\t\t  in bubble wrap and place in a small mailing envelope or container.<\/p>\n<\/li>\n<li>  Store at 4C until ready to ship.\n<\/li>\n<li>  Ship the samples with a completed requisition to Cell Line Genetics at the mailing address listed below. Ship using a 2<br \/>\n\t\t  day delivery service<\/p>\n<\/li>\n<li>  Contact Cell Line Genetics using the email address listed below to inform us of the expected delivery date (with tracking<br \/>\n\t\t  number if possible).\n\t\t  <\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>To send Suspension Cell Culture<\/strong><\/p>\n<p>&nbsp;<\/p>\n<ul style=\"padding-left:40px;\">\n<li>Transfer approximately 1 million cells to a 1.5mL microcentrifuge tube.\n<\/li>\n<li>  Label the tube with a unique cell line identifier, seal with parafilm, and place in a ziplock bag. Wrap the bag in bubble wrap.\n<\/li>\n<li>Place the sample and a filled out requisition form in a small mailing envelope or container.\n<\/li>\n<li>Store at 4C until ready to ship.\n<\/li>\n<li>Ship the samples to Cell Line Genetics at the mailing address listed below. Ship using a 2 day delivery service\n<\/li>\n<li>Contact Cell Line Genetics using the email address listed below to inform us of the expected delivery date (with tracking number if possible).\n\t\t<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>To Send Cells Frozen in Liquid Nitrogen<\/strong><\/p>\n<p>&nbsp;<\/p>\n<ul style=\"padding-left:40px;\">\n<li>Contact Cell Line Genetics for instructions\n\t\t<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>To Send Isolated DNA<\/strong><\/p>\n<p>&nbsp;<\/p>\n<ul style=\"padding-left:40px;\">\n<li>Extract genomic DNA by routine methods and resuspend DNA in water or TE buffer. CAUTION \u2013 Use of aerosol tips is highly recommended to reduce cross culture contamination during pipetting.\n<\/li>\n<li>Place at least 30uL of eluted DNA in a microcentrifuge tube. (at a concentration of at least 10ng\/uL).\n<\/li>\n<li>Make sure the top is tightly closed, and seal with parafilm. \u2022\tPlace the sample in bubble wrap inside a sealed plastic bag. Place the bag and requisition form in a small mailing envelope or container.\n<\/li>\n<li>Ship the samples to Cell Line Genetics at the mailing address listed below. Ship using a 2 day delivery service\n<\/li>\n<li>Contact Cell Line Genetics using the email address listed below to inform us of the expected delivery date (with tracking number if possible).\n\t\t  <\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<table width=\"100%\" border=\"0\" cellspacing=\"5\" cellpadding=\"2\">\n<tr>\n<td width=\"50%\"><strong>Mailing Address<\/strong><\/td>\n<td width=\"50%\"><strong>Contact Information<\/strong><\/td>\n<\/tr>\n<tr>\n<td valign=\"top\">Attention Jared Finger <br \/>\n\t\t    Cell Line Genetics <br \/>\n\t\t    510 Charmany Drive, Suite 254 <br \/>\n\t\t    Madison, WI 53719<\/td>\n<td valign=\"top\">Jared Finger <br \/>\n\t\t      Laboratory Manager <br \/>\n\t\t      608-441-8164 <br \/>\n\t\t      <a href=\"mailto:jfinger@clgenetics.com\">jfinger@clgenetics.com<\/a><\/td>\n<\/tr>\n<\/table><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>STR Profiling Services for Stem Cell Research and Regenerative Medicine STR Profiling 200 Routine QA assay to detect profile drift, mix-ups and cross contamination via STR comparison analysis\u00a0 STR200 is a quality assurance STR profile assay intended for previously authenticated cell lines. The test measures identity and detects cell line mix-ups and cross contamination by [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":114,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/clgenetics.com\/wp\/index.php?rest_route=\/wp\/v2\/pages\/166"}],"collection":[{"href":"https:\/\/clgenetics.com\/wp\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/clgenetics.com\/wp\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/clgenetics.com\/wp\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/clgenetics.com\/wp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=166"}],"version-history":[{"count":4,"href":"https:\/\/clgenetics.com\/wp\/index.php?rest_route=\/wp\/v2\/pages\/166\/revisions"}],"predecessor-version":[{"id":822,"href":"https:\/\/clgenetics.com\/wp\/index.php?rest_route=\/wp\/v2\/pages\/166\/revisions\/822"}],"up":[{"embeddable":true,"href":"https:\/\/clgenetics.com\/wp\/index.php?rest_route=\/wp\/v2\/pages\/114"}],"wp:attachment":[{"href":"https:\/\/clgenetics.com\/wp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=166"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}